Histamine release due to bivalent penicilloyl haptens: the relation of activation and desensitization of basophils to dynamic aspects of ligand binding to cell surface antibody

Journal Article · Thu Feb 01 04:00:00 EST 1979 · J. Immunol.; (United States)

OSTI ID:6394393

Dembo, M; Goldstein, B; Sobotka, A K; Lichtenstein, L M

We study the time-dependent aspects of activation and desensitization of passively sensitized human basophils by a bivalent penicilloyl hapten, (BPO)/sub 2/, in the presence or absence of a monovalent penicilloyl hapten, (BPO)/sub 1/. Data presented in this and the preceeding paper are found to be consistent with a simple kinetic model of the binding and cross-linking reaction between (BPO)/sub 1/, (BPO)/sub 2/, and cell surface IgE. It is shown that the rapid perturbation of cross-linked IgE by simple dilution or washing of cells or by addition of excess (BPO)/sub 1/ causes re-equilibration of the rate of histamine release within a matter of seconds. This demonstrates that no stable antigen-independent activated state of basophils exists and that cross-linked antibody is necessary throughout the entire time course of histamine release. In addition, we show that desensitization requires the continuing presence of cross-linked antibody on the cell surface and that the rate of desensitization is a monotone increasing function of the number of cross-linked IgE molecules per cell. The rate of desensitization also appears to be a saturable function of the number of cross-linked IgE antibodies.

• Research Organization: Los Alamos Scientific Lab., New Mexico
• OSTI ID: 6394393
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 122:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANTIBODIES
AZOLES
BASOPHILS
BINDING ENERGY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL ACTIVATION
ENERGY
HETEROCYCLIC COMPOUNDS
HISTAMINE
IMIDAZOLES
IMMUNE REACTIONS
LEUKOCYTES
LIGANDS
MATHEMATICAL MODELS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SENSITIVITY
TIME DEPENDENCE