Dissociation kinetics of the estrogen receptor immobilized by hydroxylapatite

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00508a037· OSTI ID:7056326

de Boer, W; Notides, A C

The estrogen receptor from the calf uterine cytosol was adsorbed to hydroxylapatite to provide a simpler and more controlled model for investigating in vitro the (/sup 3/H)estradiol dissociation kinetics of the estrogen receptor-nuclear complex. The dissociation of (/sup 3/H)estradiol at 29/sup 0/C from the estrogen receptor immobilized by hydroxylapatite showed biphasic kinetics with fast and slow components characteristic of the nonactivated and activated states of the receptor, respectively. The hydroxylapatite-immobilized estrogen receptor was useful in investigating the kinetics and mechanism of estrogen receptor activation and serves as a tool for understanding the estrogen receptor-nuclear interactions. (JMT)

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7056326
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 20; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CHEMICAL REACTION KINETICS
COMPLEXES
DISSOCIATION
ESTRADIOL
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
KINETICS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS