Dissociation kinetics of the nuclear estrogen receptor

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Biochemistry; (United States)

OSTI ID:6880115

de Boer, W; Notides, A C

A method was developed for measuring the (/sup 3/H)estradiol dissociation of the nuclear estrogen receptor from calf uterine nuclei that were isolated from tissue slices preincubated with (/sup 3/H)estradiol. The (/sup 3/H)estradiol dissociation from the nuclear estrogen receptor was a single, slowly dissociating component at 29/sup 0/C, k = (6.02 +/- 0.26) x 10/sup -3/ min/sup -1/. The results of the study indicate that the nuclear estrogen receptor has estradiol dissociation kinetics similar to those of the activated form of the cytoplasmic estrogen receptor and there is present a protein factor in the the uterine cytosol that affects the estrogen receptor-nuclear interaction. (JMT)

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6880115
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL REACTION KINETICS
CHROMATOGRAPHY
DISSOCIATION
ESTRADIOL
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS