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/sup 3/H-benzene metabolism in rabbit bone marrow

Journal Article · · Life Sci.; (United States)
An assay for benzene metabolism using /sup 3/H-benzene and high pressure liquid chromatography was developed. /sup 3/H-Benzene metabolism (2 pmoles benzene equivalents/mg protein/min) required the presence of a TPNH generating system and was inhibited 80% in the presence of a CO:O/sub 2/ (9:1) atmosphere. The products of /sup 3/H-benzene rabbit bone marrow microsomal metabolism were phenol and an unidentified metabolite. Cytochrome P-450 (26 to 51 pmoles/mg microsomal protein) and cytochrome c reductase activity (7.8 to 21.0 nmole/mg microsomal protein/min) were detected in rabbit bone marrow.
Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
7055876
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 25:7; ISSN LIFSA
Country of Publication:
United States
Language:
English