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Characterization and induction of aryl hydrocarbon (benzo(a)pyrene) hydroxylase in rabbit bone marrow

Journal Article · · Res. Commun. Chem. Pathol. Pharmacol.; (United States)
OSTI ID:7100338

Benzo(a)pyrene hydroxylase activity was found in the bone marrow of control and 3-methylcholanthrene(3MC)-induced New Zealand white rabbits. This activity was localized in the microsomal fraction, was NADPH dependent and CO sensitive. The reaction was inhibited by 7,8-benzoflavone indicating that it was mediated by the 3MC-inducible form of cytochrome P-450. BP hydroxylase activity in rabbit bone marrow was located primarily in white cells, and was considerably higher than that previously reported for cultured human lymphocytes and monocytes. A possible role for the bone marrow mixed-function oxygenase in the production of hemopoietic toxicity is considered.

Research Organization:
Univ. of Pennsylvania, Philadelphia
OSTI ID:
7100338
Journal Information:
Res. Commun. Chem. Pathol. Pharmacol.; (United States), Journal Name: Res. Commun. Chem. Pathol. Pharmacol.; (United States) Vol. 15:2; ISSN RCOCB
Country of Publication:
United States
Language:
English

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