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Initial organic products of fixation of (/sup 13/N)dinitrogen by root nodules of soybean (Glycine max)

Journal Article · · Plant Physiol.; (United States)
OSTI ID:7053719
; ; ; ; ;

When detached soybean Glycine max (L.) Merr. cv. Hark, nodules assimilate (/sup 13/N)N/sub 2/, the initial organic product of fixation is glutamine; glutamate becomes more highly radioactive than glutamine within 1 minute; /sup 13/N in alanine becomes detectable at 1 minute of fixation and increases rapidly between 1 and 2 minutes. After 15 minutes of fixation, the major /sup 13/N-labeled organic products in both detached and attached nodules are glutamate and alanine, plus, in the case of attached nodules, an unidentified substance, whereas (/sup 13/N)glutamine comprises only a small fraction of organic /sup 13/N, and very little /sup 13/N is detected in asparagine. The fixation of (/sup 13/N)N/sub 2/ into organic products was inhibited more than 99 percent by C/sub 2/H/sub 2/ (10 percent, v/v). The results support the idea that the glutamine synthetase-glutamate synthase pathway is the primary route for assimilation of fixed nitrogen in soybean nodules.

Research Organization:
Michigan State Univ., East Lansing
OSTI ID:
7053719
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 61:6; ISSN PLPHA
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMIDES
AMINO ACIDS
ASPARAGINE
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL ACCUMULATION
CARBOXYLIC ACIDS
CHEMISTRY
CRYOGENIC FLUIDS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
FLUIDS
FOOD
GLUTAMINE
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NITROGEN
NITROGEN 13
NITROGEN FIXATION
NITROGEN ISOTOPES
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIOISOTOPES
ROOTS
SOYBEANS
VEGETABLES