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Azolla-Anabaena relationship. XIII. Fixation of (/sup 13/N)N/sub 2/. [Azolla caroliniana; Anabaena azollae]

Journal Article · · Plant Physiol.; (United States)
OSTI ID:5905050
; ; ; ;

The major radioactive products of the fixation of (/sup 13/N)N/sub 2/ by Azolla caroliniana willd.-Anabaena azollae Stras. were ammonium, glutamine, and glutamate, plus a small amount of alanine. Ammonium accounted for 70 and 32% of the total radioactivity recovered after fixation for 1 and 10 minutes, respectively. The presence of a substantial pool of (/sup 13/N)N/sub 2/-derived /sup 13/NH/sub 4//sup +/ after long incubation periods was attributed to the spatial separation between the site of N/sub 2/-fixation (Anabaena) and a second, major site of assimilation (Azolla). Initially, glutamine was the most highly radioactive organic product formed from (/sup 13/N)N/sub 2/, but after 10 minutes of fixation glutamate had 1.5 times more radiolabel than glutamine. These kinetics of radiolabeling, along with the effects of inhibitors of glutamine synthetase and glutamate synthase on assimilation of exogenous and (/sup 13/N)N/sub 2/-derived /sup 13/NH/sub 4//sup +/, indicate that ammonium assimilation occurred by the glutamate synthase cycle and that glutamate dehydrogenase played little or no role in the synthesis of glutamate by Azolla-Azabaena.

Research Organization:
Univ. of California, Davis
OSTI ID:
5905050
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 84:3; ISSN PLPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMIDES
AMINO ACIDS
AMMONIUM COMPOUNDS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBOXYLIC ACIDS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FERNS
GLUTAMIC ACID
GLUTAMINE
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGASES
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NITROGEN 13
NITROGEN FIXATION
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHOTOSYNTHETIC BACTERIA
PLANTS
RADIOISOTOPES
REACTION KINETICS
SYMBIOSIS
SYNTHESIS
TRACER TECHNIQUES