Slowed growth of cultured fibroblasts from human radiation wounds

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Plast. Reconstr. Surg.; (United States)

DOI:https://doi.org/10.1097/00006534-198810000-00019· OSTI ID:7053188

Rudolph, R; Vande Berg, J; Schneider, J A; Fisher, J C; Poolman, W L

To study radiation effect separate from the microcirculation, fibroblasts were cultured from four patients with radiation wounds. Cells could be grown from irradiated tissue near the ulcer and from control normal tissue, but no cells could be cultured from the ulcers. The ability of radiation-treated fibroblasts to attach to the substrate and form colonies was less than that of unirradiated cells. Irradiated skin fibroblasts from the four patients had significantly longer mean generation times than did control cells. During log-phase growth (1 to 9 days), the population doublings of damaged cells were significantly reduced compared to colonies from normal cells. These data suggest a permanent intrinsic radiation effect on fibroblasts or a selective ablation of faster-growing fibroblast subpopulations that is not dependent on decreased blood supply.

• Research Organization: San Diego Veterans Administration Medical Center, La Jolla, CA (USA)
• OSTI ID: 7053188
• Journal Information: Plast. Reconstr. Surg.; (United States), Journal Name: Plast. Reconstr. Surg.; (United States) Vol. 82:4; ISSN PRSUA
• Country of Publication: United States
• Language: English

Related Subjects

560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL CULTURES
CELL DIVISION
CONNECTIVE TISSUE CELLS
FIBROBLASTS
INJURIES
MEDICINE
NUCLEAR MEDICINE
ORGANS
PATHOLOGICAL CHANGES
PATIENTS
RADIATION EFFECTS
RADIATION INJURIES
RADIOLOGY
RADIOTHERAPY
SIDE EFFECTS
SKIN
SOMATIC CELLS
THERAPY
ULCERS