Repair of DNA strand breaks in progeric fibroblasts and aging human diploid cells

Conference · Wed Jan 01 04:00:00 EST 1975

OSTI ID:4111582

Little, J B; Epstein, J; Williams, J R; Hanawalt, P C ^[1]

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1. ed.

The rate of rejoining of DNA strand breaks induced by 10 krad of $gamma$- irradiation has been studied in normal human diploid skin fibroblasts and skin fibroblasts from six patients with symptoms of progeria. Although slightly more rapid in very early passage, the repair rate in normal cells was similar throughout most of their life span in vitro. The appearance of cells with reduced repair capacity was evident as the cultures became senescent. The progeric fibroblasts varied greatly in their response to irradiation. The rate of repair was greatly reduced in two strains, whereas in two others extensive DNA degradation was consistently observed in unirradiated cells. Degradation was apparently related to the radiation received from the incorporated radiolabel. Normal repair was seen in progeric fibroblasts transformed by SV40 virus. (auth)

• Research Organization: Harvard Univ. School of Public Health, Boston
• NSA Number: NSA-33-012245
• OSTI ID: 4111582
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*DNA-- STRAND BREAKS
*FIBROBLASTS-- BIOLOGICAL RADIATION EFFECTS
*STRAND BREAKS-- RADIOINDUCTION
560121* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Cells--External Source
DISEASES
GAMMA RADIATION
MAN
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
VIRUSES