In vivo screening of candidate pretreatment compounds against cyanide using mice

Technical Report · Thu May 13 04:00:00 EDT 1993

OSTI ID:7043512

Kiser, R C; Olson, C T; Menton, R G; Hobson, D W; Moore, D M

An in vivo screening procedure was established at Battelle's Medical Research and Evaluation Facility (MREF) to evaluate the efficacy of candidate pretreatment compounds in mice challenged with the blood agent, sodium cyanide (NaCN). Male albino mice of ICR outbred stock weighing between 22.5 and 27.5 g are challenged by intramuscular (i.m.) injection, at a volume of 0.5 mL/kg, of a dose of NaCN twice the LD50 of untreated mice as determined on that day of testing. Candidate drugs are tested at fractions of their LD50 or their limit of solubility in the most optimum vehicle and given intraperitoneally (i.p.) to separate groups of mice at either 60 or 15 min prior to NaCN challenge. Sodium thiosulfate (1000 mg/kg)/sodium nitrite (100 mg/kg) controls are injected i.p. only at 60 min prior to challenge. A test compound is deemed effective if, at any of three concentrations tested, or at either pretreatment time, it is statistically more efficacious in preventing lethality than is a negative control substance (candidate compound vehicle).

• Research Organization: Battelle Memorial Inst., Columbus, OH (United States)
• OSTI ID: 7043512
• Report Number(s): AD-P-008846/8/XAB; CNN: DAMD17-83-C-3129
• Country of Publication: United States
• Language: English

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