Bioremediation of polychlorinated biphenyls: Degradation capabilities in field lysimeters

Conference · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7041386

Hill, D L; Phelps, T J; Palumbo, A V; White, D C; Strandberg, G W; Donaldson, T L

The degradation of 4-chlorobiphenyl (4CB) was compared in field lysimeters containing 60 Kg of soil contaminated with 5-10 mg/Kg of polychlorinated biphenyls. Alcaligenes A5, a bacterium carrying a plasmid for 4CB degradation, was inoculated into three lysimeters. When compared to an untreated control, soil samples from water, mineral and yeast extract treated lysimeters with and without a bacterial inoculum exhibited greater than TO fold increases in the rate of (1-/sup 14/)C-acetate incorporation into lipids and /sup 14/CO/sub 2/ production from (U-/sup 14/C-)4-chlorobiphenyl. Gene probe analyses for the 4CB plasmid and most-probable number enumerations demonstrated the presence of biodegradative populations in lysimeters and the probable survival of the added Alcaligenes A5. 17 refs., 2 figs., 2 tabs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 7041386
• Report Number(s): CONF-880521-6; ON: DE89000451
• Country of Publication: United States
• Language: English

Related Subjects

510200* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
DISSOLUTION
FIELD TESTS
HYDROCARBONS
ISOTOPE APPLICATIONS
LEACHING
LYSIMETERS
MEASURING INSTRUMENTS
MICROORGANISMS
ORGANIC COMPOUNDS
PLASMIDS
POLYCYCLIC AROMATIC HYDROCARBONS
SEPARATION PROCESSES
SOILS
TESTING
TRACER TECHNIQUES