Monitoring populations of 4-chlorobiphenyl-degrading bacterial in soil and lake water microcosms using colony hybridization
Conference
·
OSTI ID:5132680
- Tennessee Univ., Knoxville, TN (USA)
- Oak Ridge National Lab., TN (USA)
Population dynamics and maintenance of a 4-chlorobiphenyl (4CB)- degrading bacteria, Alcaligenes eutrophus, added to field lysimeters and lake water microcosms were monitored using colony hybridization. Six field lysimeters (55 gal steel drums) were set up and monitored for one year. Each lysimeter contained 60 kg of soil from a PCB-contaminated floodplain and all but one were watered and stirred during the experiment. At the start of the field lysimeter experiment, half of the lysimeters received an inoculation of Alcaligenes eutrophus. The lysimeters differed in the treatments administered over the year; some received inorganic nutrients and yeast extract and one also received biphenyl. Four 20 l microcosms were set up with water from Fort Loudon Reservoir. They were supplemented with mineral salts and glucose and stirred and monitored for two months. Alcaligenes eutrophus was inoculated into two of the microcosms, one of which was maintained with selective pressure by the addition of 4CB. One of the uninoculated microcosms also had selective pressure for 4CB-degrading organisms. 18 refs., 2 figs., 1 tab.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5132680
- Report Number(s):
- CONF-8911108-2; ON: DE90004923
- Country of Publication:
- United States
- Language:
- English
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Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50. [Alcaligenes]
Conference
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:7041386
Involvement of a chlorobenzoate-catabolic transposon, Tn5271, in community adaptation to chlorobiphenyl, chloroaniline, and 2,4-dichlorophenoxyacetic acid in a freshwater ecosystem
Journal Article
·
Tue Dec 31 23:00:00 EST 1991
· Applied and Environmental Microbiology; (United States)
·
OSTI ID:5622416
Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50. [Alcaligenes]
Journal Article
·
Tue Dec 31 23:00:00 EST 1991
· Applied and Environmental Microbiology; (United States)
·
OSTI ID:5689564
Related Subjects
54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CLEANING
CONTAMINATION
DECOMPOSITION
DECONTAMINATION
HALOGENATED AROMATIC HYDROCARBONS
LAKES
LYSIMETERS
MEASURING INSTRUMENTS
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLASMIDS
POLLUTION
POPULATION DYNAMICS
SOILS
SURFACE WATERS
WATER POLLUTION
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CLEANING
CONTAMINATION
DECOMPOSITION
DECONTAMINATION
HALOGENATED AROMATIC HYDROCARBONS
LAKES
LYSIMETERS
MEASURING INSTRUMENTS
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLASMIDS
POLLUTION
POPULATION DYNAMICS
SOILS
SURFACE WATERS
WATER POLLUTION