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The /ital polA/ gene of /ital Streptococcus pneumoniae/ and the enzyme it encodes: Cloning, expression, function and evolution

Conference ·
OSTI ID:7037486
; ; ; ;
DNA polymerase I (Pol I) of /ital E. coli/ has played an important role in elucidating the mechanisms of DNA replication and repair. Its three enzymatic activities/endash/DNA polymerase, 3'-exonuclease, and 5'-exonuclease/endash/and their roles in DNA replication and repair have been considerably investigated (Kornberg, 1980). We have cloned a gene encoding a similar DNA polymerase in /ital S. pneumoniae/, analyzed the structure and expression of the gene, and purified and characterized the pneumococcal Pol I that it encodes. We have also examined cellular functions of the enzyme in /ital S. pneumoniae/ and other bacterial hosts. Inasmuch as the Gram-positive /ital S. pneumoniae/ and the Gram-negative /ital E. coli/ are distantly related species, it is interesting to compare the structure and function of the Pol I proteins from these two sources. 17 refs., 3 figs., 1 tab.
Research Organization:
Brookhaven National Lab., Upton, NY (USA)
DOE Contract Number:
AC02-76CH00016
OSTI ID:
7037486
Report Number(s):
BNL-41705; CONF-8808128-1; BIO-4471; ON: DE88017334
Country of Publication:
United States
Language:
English

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Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL CULTURES
CLONE CELLS
CLONING
DNA POLYMERASES
DNA REPAIR
DNA REPLICATION
DNA-CLONING
ENZYMES
ESCHERICHIA COLI
GENES
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
PLASMIDS
PNEUMOCOCCUS
POLYMERASES
PURIFICATION
RECOVERY
REPAIR
STREPTOCOCCUS
TRANSFERASES