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Title: Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae

Abstract

A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.

Inventors:
; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab., Upton, NY (USA)
OSTI Identifier:
6805409
Alternate Identifier(s):
OSTI ID: 6805409
Patent Number(s):
PATENTS-US-A6090623
Assignee:
TIC; EDB-88-179643
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENES; DNA-CLONING; BACILLUS SUBTILIS; DNA POLYMERASES; DNA REPLICATION; DNA SEQUENCING; INVENTIONS; PLASMIDS; STREPTOCOCCUS; TRACER TECHNIQUES; BACILLUS; BACTERIA; CELL CONSTITUENTS; CLONING; ENZYMES; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEIC ACID REPLICATION; NUCLEOTIDYLTRANSFERASES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; STRUCTURAL CHEMICAL ANALYSIS; TRANSFERASES 550200* -- Biochemistry

Citation Formats

Lacks, S.A., Martinez, S., Lopez, P., and Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States: N. p., 1987. Web.
Lacks, S.A., Martinez, S., Lopez, P., & Espinosa, M. Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae. United States.
Lacks, S.A., Martinez, S., Lopez, P., and Espinosa, M. Fri . "Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae". United States.
@article{osti_6805409,
title = {Plasmids containing the gene for DNA polymerase I from Streptococcus pneumoniae},
author = {Lacks, S.A. and Martinez, S. and Lopez, P. and Espinosa, M.},
abstractNote = {A method is disclosed for cloning the gene which encodes a DNA polymerase-exonuclease of /und Streptococcus/ /und pneumoniae/. Plasmid pSM22, the vector containing the pneumococcal polA gene, facilitates the expression of 50-fold greater amounts of the PolI enzyme. 1 fig., 1 tab.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 28 00:00:00 EDT 1987},
month = {Fri Aug 28 00:00:00 EDT 1987}
}