Applying ion-molecule reactions to studies of gas-phase protein structure

Conference · Mon Jun 01 04:00:00 EDT 1992

OSTI ID:7036749

Ogorzalek Loo, R R; Loo, J A; Smith, R D

Whether solution phase differences in protein higher order structure persist in the gas phase, is examined by means of proton transfer reactions on ions generated by electrospray ionization of different solution conformations. Ion-molecule reactions were carried out in the atmosphere-vacuum interface of a quadrupole mass spectrometer with a Y-shaped capillary inlet-reactor. An amine (dimethyl-, trimethyl-, or diethyl-) were delivered to one inlet arm. Reactivities of bovine cytochrome c ions sprayed from denatured and native solutions were determined; the ions generated shifted to about the same charge states. Addition of equal amounts of amine to ions generated from different solution conformations of bovine ubiquitin also yielded similar final charge states; however, the average charge state increased with temperature. Myoglobin and apomyoglobin also yielded similar final charge states. The results suggest that for the non-disulfide linked proteins, either there are not significant differences in gas phase higher order structure, or proton transfer reactions are not sensitive enough to detect higher order structural differences arising from noncovalent interactions. 2 refs, 2 figs. (DLC)

• Research Organization: Pacific Northwest Lab., Richland, WA (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 7036749
• Report Number(s): PNL-SA-20437; CONF-9205196--7; ON: DE92019131
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
CARBOXYLIC ACIDS
CHARGED-PARTICLE TRANSPORT
COLLISIONS
CYTOCHROMES
DISPERSIONS
FLUIDS
GASES
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ION COLLISIONS
ION-MOLECULE COLLISIONS
MASS SPECTROMETERS
MEASURING INSTRUMENTS
MIXTURES
MOLECULE COLLISIONS
MULTIPOLES
MYOGLOBIN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEIN DENATURATION
PROTEINS
PROTON TRANSPORT
QUADRUPOLES
RADIATION TRANSPORT
SOLUTIONS
SPECTROMETERS
STRUCTURAL CHEMICAL ANALYSIS
VAPORS