Ion/ion reactions in the gas phase: Proton transfer reactions involving multiply-charged proteins

Journal Article · Wed Aug 07 04:00:00 EDT 1996 · Journal of the American Chemical Society

DOI:https://doi.org/10.1021/ja9611755· OSTI ID:374491

Stephenson, Jr, J L; MeLuckey, S A ^[1]

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1. Oak Ridge National Lab., TN (United States)

Multiply-charged cations derived from electrospray of bovine ubiquitin and horse skeletal muscle apomyoglobin have been subjected to reactions with anions derived from glow discharge ionization of perfluoro-1,3-dimethylcyclohexane. The results are compared with data obtained from proton transfer reactions with strong gaseous neutral bases. Ion/ion reaction rates are shown to be linearly related to the square of the charge on the protein ion, as expected based on a simple capture collision model. Cationic products with charge as low as +1 could be readily formed via ion/ion reactions, whereas efforts to produce such low charge states via ion/molecule reactions have proved unsuccessful. Ion/ion proton transfer reactions appear to be an effective means of reducing charge on highly charged proteins to arbitrarily low charge states. In addition to proton transfer, ion/ion recombination has also been observed. The propensity for proton transfer versus anion attachment to the cation is highly dependent upon the identity of the anion. 28 refs., 7 figs.

• DOE Contract Number: AC05-96OR22464
• OSTI ID: 374491
• Journal Information: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 31 Vol. 118; ISSN JACSAT; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
55 BIOLOGY AND MEDICINE
BASIC STUDIES
66 PHYSICS
99 GENERAL AND MISCELLANEOUS
ANIONS
CATIONS
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
GASES
MASS SPECTRA
MATHEMATICAL MODELS
MYOGLOBIN
PROTEINS
PROTONS