High-resolution mapping of probes near the X-linked lymphoproliferative disease (XLP) locus
Journal Article
·
· Genomics; (United States)
- Boston Univ. School of Medicine, MA (United States)
- Baylor College of Medicine, Waco, TX (United States)
- Johns Hopkins Univ. Medical School, Baltimore, MD (United States)
- Washington Univ. School of Medicine, Seattle, WA (United States)
Fluorescence in situ hybridization (FISH) was employed in high-resolution mapping of probes near the X-linked lymphoproliferative disease (XLP) locus. The map includes the DXS42, DXS12, DXS6, DXS982, DXS739, DXS75, DXS100, DXS10, and DXS177 loci. Metaphase analysis showed that DXS1 and DXS42 mapped to proximal Xq25, while DXS10 and DXS177 mapped to proximal Xq26.1. DXS6, DXS982, DXS739, DXS75, and DXS100 were in Xq25. The order of probes deduced from interphase FISH was: Xq24-(DXS12, DXS42)-DXS6-DXS982-DXS739-DXS75-DXS100-DXS10-DXS177-Xq26.2. The authors estimate that the entire region between DXS12 and DXS177 is about 7 Mb. A previous study indicated that all three XLP deletions (63.3, 66-1, and 43-4) lacked DXS739. They now report that DXS75 and DXS982 are also missing in these deletions. Using interphase FISH measurements, they estimate that 2 Mb are absent in 63-3, and 4 Mb are absent in 66-1 and 43-4. This FISH map confines the XLP candidate gene region to a 2-Mb interval between DXS6 and DXS100 and places DXS100 distal to the XLP locus. This study also demonstrates that small probes (0.6 to 3.5 kb) can be utilized in FISH. 29 refs., 5 figs., 4 tabs.
- OSTI ID:
- 7035519
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:1; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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