Organization of the multiple polymorphic sites of the D19S11 locus within a 650-kb cosmid contig

Journal Article · Sun Aug 01 04:00:00 EDT 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1327· OSTI ID:7035444

Tynan, K; Hoffman, S M.G.; Tsujimoto, S; Brandriff, B; Gordon, L; Carrano, A V; Mohrenweiser, H W ^[1]

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1. Lawrence Livermore National Lab., CA (United States)

The D19S11 locus has been previously described as consisting of a complex set of six nonallelic polymorphic sites detected with a combination of four restriction enzymes and three probes that were subcloned from a single cosmid. These probes also hybridized to additional nonvariant fragments on Southern blots of human genomic DNA. In the course of establishing a contig map of human chromosome 19, a set of cosmids that were positive for at least one of the probes defining this locus was identified. These cosmids, along with additional cosmids, were assembled using a combination of strategies, including fluorescence in situ hybridization studies using G1 interphase nuclei and sperm pronuclei as chromatin targets, into a single overlapping set of cosmids that spans [approximately]650 kb. Cosmids that are positive for the MEL gene probe are localized at the centromeric end of the spanning path, with some cosmids being positive for both the MEL gene probe and one of the D19S11 probes. The EcoRI fragments with homology to the various probes have been identified; some cosmids have homology to all three D19S11 probes. The positions for five of the six polymorphic sites were localized within a 40-kb region, with four sites within 15 kb. 21 refs., 3 figs., 2 tabs.

• OSTI ID: 7035444
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 17:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
COSMIDS
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOME 19
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING