A YAC, P1, and cosmid contig and 17 new polymorphic markers for the Werner syndrome region at 8p12-p21
- Veterans Affairs Puget Sound Health Care System, Seattle, WA (United States)
- Univ. of Washington, Seattle, WA (United States); and others
A yeast artificial chromosome (YAC), P1, and cosmid clone contig was constructed for the Werner syndrome (WRN) region of chromosome 8p12-p21 and used to clone a candidate gene for WRN. This region also possibly contains a familial breast cancer locus. The contig was initiated by isolating YACs for the glutathione reductase (GSR) gene and extended in either direction by walking techniques. Sequence-tagged site (STS) markers were generated from subclones of 2 GSR YACs and used to identify P1 and cosmid clones. Additional STSs were generated from P1 and cosmid clones and from potential expressed sequences identified by cDNA selection and exon amplification methods. The final contig was assembled by typing 17 YACs, 20 P1 clones, and 109 cosmids for 54 STS markers. The WRN region could be spanned by 2 nonchimeric YACs covering approximately 1.4 Mb. A P1/cosmid contig was established covering the core 700-800 kb of the WRN region. Fifteen new short tandem repeat polymorphisms and 2 biallelic polymorphic markers were identified and included as STSs in the contig. Analysis of these markers in Werner syndrome subjects demonstrates that the candidate WRN gene is in a region of linkage disequilibrium. 47 refs., 2 figs., 3 tabs.
- DOE Contract Number:
- FG06-93ER61553
- OSTI ID:
- 465977
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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BASIC STUDIES
AGING
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
COSMIDS
DESIGN
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 8
IN-SITU HYBRIDIZATION
PATIENTS
PHENOTYPE
RADIATION INDUCED MUTANTS
RECESSIVE MUTATIONS