A 4.5-megabase YAC contig and physical map over the hemochromatosis gene region

Journal Article · Mon Apr 15 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0178· OSTI ID:465943

Burt, M J; Smit, D J; Pyper, W R; Powell, L W; Jazwinska, E C ^[1]

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1. Univ. of Queensland, Brisbane (Australia)

We have constructed a yeast artificial chromosome (YAC) contig over the candidate hemochromatosis gene region. This contig comprises hemochromatosis gene region. This contig comprises 16 YACs from the CEPH, Washington University, and ICI YAC libraries and covers 4.5 Mb at 6p21.3-6p22. The complete contig has been restriction mapped, enabling the precise relationship between the YACs to be determined and the mapping of a total of 12 STSs. Nine of these are highly polymorphic STSs that are closely linked to hemochromatosis; this series includes D6S265 and D6S1260, which comprise the most proximal and distal markers linked to HC. This is the first YAC contig that spans the hemochromatosis candidate region, and it provides valuable resource material for the cloning of this and other genes in the region distal to the MHC class I complex. 33 refs., 1 fig., 1 tab.

• OSTI ID: 465943
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 33; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CONTIGS
DESIGN
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 6
IN-SITU HYBRIDIZATION
IRON
MAN
METABOLIC DISEASES
METABOLISM
RECESSIVE MUTATIONS
TRANSCRIPTION