Assay for pyridoxal phosphate in 10 microliters of human venous and capillary blood plasma

Journal Article · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7035405

Howard, M P; Andon, M B; Reynolds, R D

Plasma pyridoxal phosphate (PLP) concentrations of venous (V), capillary (C) and V-blood drawn into a hematocrit (Hct) tube (VH) and processed exactly as the C-blood were determined by the standard (STD) or a modified micro (MCR) tyrosine apodecarboxylase procedure. Compared to the STD the MCR uses 20 times the spec. act. of /sup 14/C-tyrosine, a 40 vs 20 min reaction time and 10 vs 850 ..mu..l plasma. Conclusions indicate that STD and MCR generate equivalent values. C-plasma gives slightly higher PLP concentrations than V-plasma which may be related to packing of blood cells in the Hct tube.

• Research Organization: Dept. of Agriculture, Beltsville, MD
• OSTI ID: 7035405
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BLOOD COUNT
BLOOD PLASMA
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
ENZYMES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYASES
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
TRACER TECHNIQUES
TYROSINE