Genes in one megabase of the HLA class I region
- Yale Univ. School of Medicine, New Haven, CT (United States)
- Yale Univ. School of Medicine, New Haven, CT (United States) R.W. Johnson Pharmaceutical Research Institute, Raritan, NJ (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
To define the gene content of the HLA class I region, cDNA selection was applied to three overlapping yeast artificial chromosomes (YACs) that spanned 1 megabase (Mb) of this region of the human major histocompatibility complex. These YACs extended from the region centromeric to HLA-E to the region telomeric to HLA-F. In additions to the recognized class I genes and pseudogenes and the anonymous non-class-I genes described recently by the authors and others, 20 additional anonymous cDNA clones were identified from this 1-Mb region. They also identified a long repetitive DNA element in the region between HLA-B and HLA-E. Homologues of this outside of the HLA complex. The portion of the HLA class I region represented by these YACs shows an average gene density as high as the class II and class III regions. Thus, the high gene density portion of the HLA complex is extended to more than 3 Mb.
- OSTI ID:
- 7034328
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 90:22; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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