An improved method for producing radiation hybrids applied to human chromosome 19

Technical Report · Wed Jan 01 04:00:00 EST 1992

DOI:https://doi.org/10.2172/7032682· OSTI ID:7032682

Jackson, C L; Mark, H F.L.

Using radiation hybrids from a monochromosomal microcell hybrid containing human chromosome 19 as its only human component (PK87-19), we have initiated analysis of a panel of hybrids for markers in known locations on human chromosome 19. Also begun was a fluorescent in situ hybridization analysis of the hybrid cell lines using biotinylated total human DNA as a hybridization probe to metaphase chromosomes prepared from the hybrids cell lines. We are analyzing our panel of 94 hybrids for additional markers obtained from the literature, or the genome data base as well as to complete the analysis of any hybrids not yet scored for the markers iii the table. The hybrid panel has been tested for apolipoprotein C{sub 2} for the radiation hybrids for D19Sl77 (mfd 120), D19Sl78 (mfd 139) and for HRC (histidine rich calcium binding protein). In addition we have also analyzed for the presence of slow troponin 1 (TNNT1) and GPI (glucose phosphate isomerase).

• Research Organization: Rhode Island Hospital, Providence, RI (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER61136
• OSTI ID: 7032682
• Report Number(s): DOE/ER/61136-2; ON: DE93003156
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOMES
DOCUMENT TYPES
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
GENE MUTATIONS
HUMAN CHROMOSOME 19
HUMAN CHROMOSOMES
MUTANTS
MUTATIONS
PROGRESS REPORT
RADIATION INDUCED MUTANTS
SPECTROSCOPY