An improved method for producing radiation hybrids applied to human chromosome 19. Technical progress report, March 1, 1991--February 28, 1992
At the initiation of the grant we had just produced radiation hybrids from a monochromosomal microcell hybrid containing human chromosome 19 as its only human component. Radiation hybrids were produced using doses of radiation ranging from 1000--8000 rads. Lethally irradiated cells were then fused to hamster recipients (CHTG49) and selected for growth in histidinol. Approximately 240 clones were isolated and 75 clones were expanded for the isolation of DNA. This report describes in situ hybridization studies and the introduction of markers into human chromosome 19.
- Research Organization:
- Rhode Island Hospital, Providence, RI (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-91ER61136
- OSTI ID:
- 10136386
- Report Number(s):
- DOE/ER/61136--1; ON: DE92011030
- Country of Publication:
- United States
- Language:
- English
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