Biodistribution of monoclonal antibody B72. 3(I-131) in colon cancer patients

Conference · Wed May 01 00:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:7031967

Colcher, D; Carrasquillo, J A; Sugarbaker, P; Bryant, G; Esteban, J; Simpson, D; Thor, A; Reynolds, J R; Horowitz, M; Keenan, A M

Monoclonal antibody (MAb) B72.3 was generated against a carcinoma metastasis and has been shown to bind with a high degree of selectivity to a tumor associated glycoprotein (TAG-72) with a molecular weight of greater than 200,000 dal. The TAC-72 antigen in present on greater than 80% of human colon carcinomas when tested by immunoperoxidase with no significant reactivity to normal adult tissues. MAb B72.3 was radiolabeled with I-131 and administered intravenously to patients with metastatic colon cacinomas. The amount of MAb B72.3 injected was varied from 0.16-3.95 mg (0.8-10mCi of I-131) without significantly affecting the biodistribution and clearance. Positive scans were obtained in 10 of 19 patients. Most of the patients studied were scheduled for surgical procedures 7-14 days post injection of the MAb allowing us to verify the biodistribution of the MAb. Radioactivity per gram was determined and tumor to normal tissue ratios calculated; these ratios varied from 2:1 to 38:1 in the patients studied. In most lesions the tumor to tissue ratios were greater than 5:1 when compared to any of the normal tissues assayed. At the time of surgery the tumors retained up to 0.01% of the injected dose per gram. Autoradiography confirmed the specificity of the uptake of the I-131 MAb in the tumor masses. The specificity of the localization of MAb B72.3 in vivo demonstrates the potential of this antibody as a diagnostic agent and as a therapeutic agent.

• Research Organization: Lab. of Tumor Immunology and Biology, Surgery Branch, Lab. of Pathology, NCI, NIH, Bethesda, MD
• OSTI ID: 7031967
• Report Number(s): CONF-850611-
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

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ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CARBOHYDRATES
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DOSIMETRY
DRUGS
GASTROINTESTINAL TRACT
GLUCOPROTEINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 131
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LARGE INTESTINE
MEDICINE
METABOLISM
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPES
RADIOLOGY
RADIOPHARMACEUTICALS
RADIOTHERAPY
SACCHARIDES
SURGERY
THERAPEUTIC USES
THERAPY
TISSUE DISTRIBUTION
UPTAKE
USES