Radioimmunoscintigraphy of human colon cancer xenografts in mice with radioiodinated monoclonal antibody B72. 3

Journal Article · Thu Nov 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:5989843

Keenan, A M; Colcher, D; Larson, S M; Schlom, J

Monoclonal antibody B72.3 reacts with a tumor-associated antigen that is found on human breast and colon carcinomas in significantly higher concentration than in normal adult tissues. Intact B72.3 lgG was labeled with I-131 or I-125 and injected into athymic mice bearing xenografts of human colon cancer. Whole-body scintiphotos obtained with a pinhole collimator demonstrated tumor localization within 24 hr after intravenous administration, and the tumor-to-background ratio rose continuously for at least 14 days. Progressive antibody accumulation was observed in the tumor during the first 3 days, but no significant normal organ localization was observed at any time. No localization was seen in control tumors, a human melanoma xenograft that lacks the antigen recognized by B72.3. The pharmacokinetics of this antibody in tumor-bearing mice suggest that I-131 B72.3 may be useful for radioimmunotherapy as well as radioimmunoscintigraphy of colon cancer in man.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5989843
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:11; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GRAFTS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LARGE INTESTINE
MAMMALS
MAN
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PRIMATES
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SCINTISCANNING
TRANSPLANTS
USES
VERTEBRATES