Solid phase exchange labeling of I-123-HIPDM

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:7030510

Mangner, T; Rogers, W L; Wieland, D; Clinthorne, N; Juni, J; Koral, K; Mayans, R; Swanson, D

Reported brain uptake of the I-123 diamine, HIPDM, ranges from 3.5% to 8.5% of the injected dose. This variability has been attributed to varying purity of the labeled compound produced by published methods. The authors have obtained excellent radioiodide incorporation into HIPDM using a simple, 5-minute, solid-phase exchange reaction in the presence of ammonium sulfate or bisulfate. However, when ammonium sulfate was used, HPLC analysis (..mu..Bodapak-phenyl; 1% ammonium acetate (pH 5.5)/methanol, 30/70) revealed the presence of, in addition to labeled HIPDM and unreacted radioiodide, two additional labeled compounds, each comprising 4% of the total activity. Lowering the initial pH of the reaction by the use of ammonium bisulfate resulted in quantitative radioiodide incorporation to give chromatographically pure HIPDM. To date, this preparation has been tested in one normal human. Percent uptake in brain was determined by tomographic imaging with both a single photon ring tomograph (SPRINT) and rotating Anger camera (GE 400-T). In each case high energy background was determined by acquiring data with lead shielding over the collimator. This background was then subtracted from the images. Calibration was performed by imaging a 19 cm diameter uniform cylindrical phantom filled with I-123. Both sets of images were attenuation corrected with the Sorenson method. Total uptake in the brain was between 6.7% and 7.2% depending upon selection of the brain outline. This agrees with values reported previously and is quite comparable to those measured for iodoamphetamine.

• Research Organization: Univ. of Michigan Medical Center, Ann Arbor, MI
• OSTI ID: 7030510
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ACETATES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
AMMONIUM SULFATES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CAMERAS
CARBOXYLIC ACID SALTS
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
IMPURITIES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
METHANOL
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PH VALUE
PRIMATES
QUANTITY RATIO
RADIOISOTOPES
SEPARATION PROCESSES
SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY
SULFATES
SULFUR COMPOUNDS
TOMOGRAPHY
TRACER TECHNIQUES
UPTAKE
USES
VERTEBRATES