Technetium-99m-labeled para aminohippuric acid analog: A new renal agent
A renal agent which is labelled with Tc-99m and quantitatively secreted by the tubules has been sought unsuccessfully for many years. The authors have successfully labelled a PAH analog, synthesized by the coupling of p-aminohippuric acid with nitrilotriacetic acid anhydride. The cold compound (PAHIDA) is characterized by elemental analysis, mass spectroscopy, NMR and HPLC techniques and yields a stable complex with Tc-99m at a pH of 5.8 using the Sn (II) reduction method. The quality control and the stability of the Tc-99m complex has been established by ITLC and HPLC techniques. Biodistribution of the Tc-99m-PAHIDA was determined in mice and rats at different time intervals and compared with I-131-hippuran. The percent administered dose present at 30 minutes after injection in mice in blood, kidneys, urine and liver respectively is 1.3 +- 0.4, 1.4 +- 0.4, 79.4 +- 6.0, and 0.7 +- 0.2, n=6. Biodistribution values in rats at thirty minutes post injection for blood, kidneys, urine and liver respectively are 6.3 +- 1.0, 4.4 +- 0.7, 61.3 +- 12.1, and 1.2 +- 0.3 for Tc-99m-PAHIDA and 1.2 +- 0.2, 0.9 +- 0.2, 64.8 +- 2.0 and 0.9 +- 0.3 for I-131-hippuran. Rat urine analysis by paper chromatography suggest that the agent is excreted intact without any metabolic alteration. Scintigraphic images in rabbits have shown that the Tc-99m labeled agent is rapidly excreted in urine with no significant extrarenal pathway providing excellent renal images. The reported agent Tc-99m-PAHIDA satisfies structural requirements for renal secretion owing to the presence of R-CO-NH-CH/sub 2/-COOH grouping analogous to hippuran and may have the potential to replace I-131 hippuran for renal studies with high target to background.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY
- OSTI ID:
- 7030346
- Report Number(s):
- CONF-840619-; TRN: 87-010808
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENINES
DIAGNOSTIC USES
LABELLING
KIDNEYS
SCINTISCANNING
ACETIC ACID
HIPPURIC ACID
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MICE
NMR SPECTRA
PH VALUE
POLYCYCLIC AROMATIC HYDROCARBONS
QUALITY CONTROL
RATS
SECRETION
STABILITY
TECHNETIUM 99
TRACER TECHNIQUES
AMINES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CONTROL
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DRUGS
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
HYDROCARBONS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PURINES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
TECHNETIUM ISOTOPES
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics