Technetium-99m-labeled para aminohippuric acid analog: A new renal agent
A renal agent which is labelled with Tc-99m and quantitatively secreted by the tubules has been sought unsuccessfully for many years. The authors have successfully labelled a PAH analog, synthesized by the coupling of p-aminohippuric acid with nitrilotriacetic acid anhydride. The cold compound (PAHIDA) is characterized by elemental analysis, mass spectroscopy, NMR and HPLC techniques and yields a stable complex with Tc-99m at a pH of 5.8 using the Sn (II) reduction method. The quality control and the stability of the Tc-99m complex has been established by ITLC and HPLC techniques. Biodistribution of the Tc-99m-PAHIDA was determined in mice and rats at different time intervals and compared with I-131-hippuran. The percent administered dose present at 30 minutes after injection in mice in blood, kidneys, urine and liver respectively is 1.3 +- 0.4, 1.4 +- 0.4, 79.4 +- 6.0, and 0.7 +- 0.2, n=6. Biodistribution values in rats at thirty minutes post injection for blood, kidneys, urine and liver respectively are 6.3 +- 1.0, 4.4 +- 0.7, 61.3 +- 12.1, and 1.2 +- 0.3 for Tc-99m-PAHIDA and 1.2 +- 0.2, 0.9 +- 0.2, 64.8 +- 2.0 and 0.9 +- 0.3 for I-131-hippuran. Rat urine analysis by paper chromatography suggest that the agent is excreted intact without any metabolic alteration. Scintigraphic images in rabbits have shown that the Tc-99m labeled agent is rapidly excreted in urine with no significant extrarenal pathway providing excellent renal images. The reported agent Tc-99m-PAHIDA satisfies structural requirements for renal secretion owing to the presence of R-CO-NH-CH/sub 2/-COOH grouping analogous to hippuran and may have the potential to replace I-131 hippuran for renal studies with high target to background.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY
- OSTI ID:
- 7030346
- Report Number(s):
- CONF-840619-
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 25:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETIC ACID
ADENINES
AMINES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CONTROL
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DRUGS
HETEROCYCLIC COMPOUNDS
HIPPURIC ACID
HOURS LIVING RADIOISOTOPES
HYDROCARBONS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MASS SPECTROSCOPY
MICE
MONOCARBOXYLIC ACIDS
NMR SPECTRA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PH VALUE
POLYCYCLIC AROMATIC HYDROCARBONS
PURINES
QUALITY CONTROL
RADIOISOTOPE SCANNING
RADIOISOTOPES
RATS
RODENTS
SCINTISCANNING
SECRETION
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
STABILITY
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES