Comparison of sister chromatid exchange induction in murine germinal and somatic cells by gamma radiation exposure in vivo

Journal Article · Sat Dec 31 04:00:00 EST 1994 · Environmental and Molecular Mutagenesis

DOI:https://doi.org/10.1002/em.2850240203· OSTI ID:70297

Morales-Ramirez, P; Mendiola-Cruz, M T; Vallarino-Kelly, T; Rodriguez-Reyes, R ^[1]

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1. Instituto Nacional de Investigaciones Nucleares, Lomas Barrilaco (Mexico)

Sister chromatid exchange (SCE) induction by gamma rays was determined in speratogonia irradiated before or after BrdU incorporation. Furthermore, the comparison of responses obtained in spermatogonia, bone marrow and salivary gland cells was carried out in the cells irradiated after BrdU incorporation, a condition which permits a higher SCE induction. Results indicate that gamma ray exposure of spermatogonia could induce a significant increase in SCE frequency with doses as low as 0.27 Gy, either before or after BrdU incorporation. However, the increase caused by radiation exposure after BrdU incorporation in spermatogonia was nearly three times lower than that obtained in both bone marrow and salivary gland cells. These data suggest that spermatogonia are either more efficient in repairing the gamma ray-induced lesions involved in SCE production or that these cells are less prone to the induction of such lesions. 53 refs., 2 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 70297
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: 2 Vol. 24; ISSN EMMUEG; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANIMAL CELLS
CHROMOSOMAL ABERRATIONS
GAMMA RADIATION
GENETIC RADIATION EFFECTS
IN VIVO
SOMATIC CELLS
SPERMATOGONIA