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Effect of [sup 60]Co [gamma]-irradiation on the nonspecific cytotoxicity of alveolar macrophages in vitro

Journal Article · · Environmental Health Perspectives; (United States)
DOI:https://doi.org/10.2307/3431348· OSTI ID:7028423
; ;  [1]
  1. Institute of Radiation Medicine, Beijing (China)
+ Show Author Affiliations
This paper reports on the effect of radiation on the nonspecific cytotoxicity of rat alveolar macrophages (AM). AM (effector cells) of bacille Calmette-Guerin-activated Wistar rats were irradiated with [sup 60]Co [gamma] rays in vitro to give doses of 0, 100, 300, and 500 Gy. Three hours after irradiation, the AM were cultured with human lung adenocarcinoma AGZY83-a and HeLa target cells in [sup 125]I-deoxyuridine-containing media for 6 hr and the cytotoxicity indexes determined. The results indicated that the cytotoxicity indexes of AM against human lung adenocarcinoma cells and HeLa cells were 94.3 [+-] 0.3% and 81.3 [+-] 1.9%, respectively. The cytotoxicity indexes using an effector/target cell ratio (E/T) of 10 seemed to be greater than with ratios of 20 and 30. The cytotoxicity indexes of AM (7 rats), irradiated to give doses of 0, 100, 300, and 500 Gy, against adenocarcinoma cells at an E/T ratio of 10 were 87.9 [+-] 8.4%, 65.4 [+-] 14.1%; 47.5 [+-] 17.5%, and 36.7 [+-] 9.7%, respectively. The significance of the nonspecific cytotoxicity of AM in the immunological elimination of tumors and the inhibitory effect of radiation on AM cytotoxicity are discussed. 6 refs., 2 figs., 4 tabs.
OSTI ID:
7028423
Journal Information:
Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 97; ISSN EVHPAZ; ISSN 0091-6765
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550603* -- Medicine-- External Radiation in Therapy-- (1980-)
550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOMAS
COBALT 60
COBALT ISOTOPES
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DEOXYURIDINE
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EVALUATION
GAMMA RADIATION
HELA CELLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MACROPHAGES
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RATS
RIBOSIDES
RODENTS
SOMATIC CELLS
URACILS
VERTEBRATES
YEARS LIVING RADIOISOT