delta. 5 desaturation of fatty acids in L-M cells

Journal Article · Tue Feb 28 04:00:00 EST 1978 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(78)91317-7· OSTI ID:7027404

Lambremont, E N ^[1]; Lee, T C; Blank, M L; Snyder, F

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1. Oak Ridge Associated Univs., TN

L-M cells grown in a lipid-free medium containing /sup 14/C-labeled 9, 12-linoleic acid incorporated most of this acid into glycerolipids as linoleic acid. Only a small amount (3%) was elongated to eicosadienoic acid. No ..delta..6 desaturation occurred. When the cells were incubated with /sup 14/-C-labeled 8, 11, 14-eicosatrienoic acid, 22% of the activity was found in 5, 8, 11, 14-eicosatetraenoic acid. Treatment of the cells for 24 hr with N-isopropylethanolamine, a choline analog, depressed this desaturation reaction to about 60% of control values. The identity of the tetraene product was established by two different chromatographic analyses of the fatty acid methyl esters. Location of the double bond at position C-5 was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas-liquid chromatography. These results prove that L-M cells have a ..delta..5 desaturase and an elongation enzyme converting 18 : 2 to 20 : 2, but lack a ..delta..6 desaturase.

• OSTI ID: 7027404
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 80:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CELL CULTURES
CHEMICAL BONDS
CHEMISTRY
CHOLINE
CHROMATOGRAPHY
DOUBLE BONDS
EICOSANOIC ACID
ENZYMES
ESTERS
EVEN-EVEN NUCLEI
HYDROXY COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LINOLEIC ACID
LIPIDS
LIPOTROPIC FACTORS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
QUATERNARY COMPOUNDS
RADIOISOTOPES
SEPARATION PROCESSES
YEARS LIVING RADIOISOTOPES