Desaturation and chain elongation of essential fatty acids in isolated liver cells from rat and rainbow trout

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534822· OSTI ID:5528529

Hagve, T A; Christophersen, B O; Dannevig, B H

Isolated hepatocytes from rainbow trout and rat were incubated with /sup 14/C-labeled linoleic acid, linolenic acid, dihomogammalinolenic acid or eicosapentaenoic acid. The most striking difference in the desaturase activity was the lower level of delta 5 desaturase in trout than in rat. No delta 4 desaturation of 22:4(n-6) to 22:5(n-6) was observed in either of the two species, while the conversion of 22:5(n-3) to 22:6(n-3) was significant in both groups and highest in rainbow trout. The chain-elongating activity was remarkably similar in the two species, except for the dead-end elongation which was distinctly more important in fish.

• Research Organization: Univ. of Oslo, Rikshospitalet, Norway
• OSTI ID: 5528529
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 3; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
EICOSANOIC ACID
ENZYME ACTIVITY
ENZYMES
ESTERS
FISHES
KINETICS
LABELLED COMPOUNDS
LABELLING
LINOLEIC ACID
LIPIDS
LIVER CELLS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHOSPHOLIPIDS
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
TROUT
VERTEBRATES