Biological studies of the control of rat hepatocyte proliferation in primary culture

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:7027088

Koch, K S

The goals of this study were, (1) to develop defined conditions for hepatocyte growth reinitiation bioassays in vitro; (2) to use these bioassays to identify mitogens that might regulate regeneration in vivo; (3) to study interactions among mitogens; and (4) to analyze mechanisms of mitogen action. Bioassays in serum-free media were developed with fetal and adult hepatocytes using enrichment and primary monolayer culture techniques. Prominent differentiated functions, including alpha-fetoprotein and albumin biosynthesis, were monitored biochemically with specific antisera. Proliferation was quantified by cell counting, determination of rates of (/sup 3/H) thymidine incorporation into DNA, autoradiographic determination of (/sup 3/H) thymidine nuclear labelling and mitotic indices, chemical determination of DNA content, and flow microfluorometry. Standard radioisotopic labelling procedures were used to monitor RNA and protein synthesis, amino acid transport, and ion fluxes. Cellular sodium and calcium contents were measured by flame photometry and atomic absorption spectroscopy, respectively.

• Research Organization: California Univ., San Diego (USA)
• OSTI ID: 7027088
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTRA
ALBUMINS
ANIMAL CELLS
AZINES
BIOASSAY
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CELL PROLIFERATION
DNA
FUNCTIONS
GROWTH
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER CELLS
MITOGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
SOMATIC CELLS
SPECTRA
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS