Developmental changes in myelin-induced proliferation of cultured Schwann cells

Journal Article · Sun Mar 01 04:00:00 EST 1987 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.104.3.655· OSTI ID:6863399

Yoshino, J E; Mason, P W; DeVries, G H

Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated (/sup 3/H)thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with (/sup 14/C)thymidine followed by a pulse of (/sup 3/H)thymidine 24 h later, every Schwann cell labeled with (/sup 3/H)thymidine was also labeled with (/sup 14/C)thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.

• Research Organization: Medical College of Virginia, Richmond
• OSTI ID: 6863399
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 3; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CELL PROLIFERATION
DNA REPLICATION
HETEROCYCLIC COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MITOGENS
MYELIN
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES