Interleukin 6 stimulates hepatic glucose release from prelabeled glycogen pools

Journal Article · Mon Jan 01 00:00:00 EST 1990 · American Journal of Physiology; (USA)

OSTI ID:7026900

Ritchie, D G ^[1]

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1. Shriners Burns Institute, Galveston, TX (USA)

Cytokines, derived from a wide variety of cell types, are now believed to initiate many of the physiological responses accompanying the inflammatory phase that follows either Gram-negative septicemia or thermal injury. Because hypoglycemia (after endotoxic challenge) and hyperglycemia (after thermal injury) represent well-characterized responses to these injuries, we sought to determine whether hepatic glycogen metabolism could be altered by specific cytokines. Cultured adult rat hepatocytes were prelabeled with ({sup 14}C)glucose for 24 h, a procedure that resulted in the labeling of hepatic glycogen pools that subsequently could be depleted (with concomitant ({sup 14}C)glucose release) by either glucagon or norepinephrine. After the addition of a highly concentrated human monocyte-conditioned medium (MCM) or various cytokines to these prelabeled cells, ({sup 14}C)glucose release was stimulated by MCM and recombinant human interleukin 6 (IL-6) but was not stimulated by other cytokines tested. Furthermore, only antisera to IL-6 were capable of reducing the glucose-releasing factor activity found in MCM. These data therefore suggest a novel glucoregulatory role for IL-6.

• OSTI ID: 7026900
• Journal Information: American Journal of Physiology; (USA), Vol. 258; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
LYMPHOKINES
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CULTURE MEDIA
GLUCAGON
GLYCOGEN
GROWTH FACTORS
HEMIC DISEASES
HYPERGLYCEMIA
INTERFERON
LIVER
MONOCYTES
RATS
TRACER TECHNIQUES
TRYPSIN
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
DIGESTIVE SYSTEM
DISEASES
ENZYMES
GLANDS
HEXOSES
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
POLYSACCHARIDES
PROTEINS
REACTION KINETICS
RODENTS
SACCHARIDES
SERINE PROTEINASES
VERTEBRATES
550501* - Metabolism- Tracer Techniques