Electrophoretic shift mutants in Chinese hamster ovary cells: evidence for genetic diploidy
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Electrophoretic shift mutants induced in Chinese hamster ovary (CHO) cells indicate that these cells are not extensively functionally hemizygotic. Therefore, effective haploidy is unsatisfactory as a general theory to explain the frequency of recessive mutants in this cell line. CHO cells were screened for electrophoretic shift variants of enzymes coded by approximately 40 genetic loci. Clones isolated after exposure to ultraviolet radiation were examined by starch gel and Cellogel electrophoresis. Shift variants were recovered for enzymes representing 11 different loci. Variant clones were subcloned to demonstrate the heritability of the variations. Mutants at nine loci produced multiple-banded patterns consistent with the patterns expected of genes at loci represented twice (diploid). Chromosome localization of these diploid loci in other mammalian species where they have been mapped, suggests that they represent a random sample of CHO genes. Chromosome analysis of mutant subclones indicated that the variation did not take place in tetraploid cells. The data indicate that the quasi-diploid CHO cells appear only as functionally hemizygous as would be expected of a slightly hypodiploid cell line derived from an organism in which the haploid number is 11.
- Research Organization:
- Univ. of Texas System Cancer Center, Houston
- OSTI ID:
- 7026129
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 75:4; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
CELL CULTURES
DIPLOIDY
ELECTROMAGNETIC RADIATION
ENZYMES
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENETIC VARIABILITY
HAMSTERS
IRRADIATION
MAMMALS
MUTATIONS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECESSIVE MUTATIONS
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
CELL CULTURES
DIPLOIDY
ELECTROMAGNETIC RADIATION
ENZYMES
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GENETIC VARIABILITY
HAMSTERS
IRRADIATION
MAMMALS
MUTATIONS
PLOIDY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECESSIVE MUTATIONS
RODENTS
ULTRAVIOLET RADIATION
VERTEBRATES