Effects of anaerobiosis on in vivo protein synthesis in the roots of a marine angiosperm zostera marina

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:7025796

Smith, R D; Alberte, R S ^[1]

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1. Univ. of Chicago, IL (USA)

The roots of the temperate seagrass Zostera marina undergo daily periods of anaerobiosis at night. These diurnal periods of anoxia alter many metabolic processes in the roots including carbon and nitrogen metabolism, amino acid synthesis, and synthesis and levels of ATP, ADP and AMP. To further characterize the effects of anaerobiosis, we determined in vivo rates of protein synthesis by measuring the relative incorporation of {sup 35}S-MET in TCA precipitated protein samples. Results from these studies show that in vivo protein synthesis decreases continuously during 12 h of anaerobiosis and correlates with changes in ATP levels under similar conditions. Furthermore, polypeptide patterns obtained by SDS-PAGE and 2D-SDSPAGE indicate that anaerobiosis leads to differential protein synthesis in the roots.

• OSTI ID: 7025796
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANOXIA
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES