Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:7025769

Rodriguez-Campos, E; Bernal-Lugo, I; Hamabata, A ^[1]

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1. CINVESTAV-IPN (Mexico)

Wheat aleurone has the capacity of acidifying the incubation medium in 1 to 2 pH units. The {alpha}-amylase induction by GA{sub 3} in isolated wheat aleurone layers is strongly dependent on acidic pH of the medium (pH < 5). To examine possible mechanisms {sup 35}-Met incorporation into proteins and {alpha}-amylase, in the presence of GA{sub 3} and Ca{sup 2+} at pH, 4, 5 and 6 was studied. Although {sup 35}-Met uptake decreased markedly ({approx} 90%) at pH 4 in thepresence of GA{sub 3}, incorporation into total protein did not change significantly from other conditions. Auto-radiography of SDS-PAGE showed that most of the amino acid was in the {alpha}-amylase band, meaning that the effect of acidic pH is specific for GA{sub 3} actions on aleurone tissue. On the other hand, an increase of protonated GA{sub 3} diffusion could be ruled out. Also, there was not {alpha}-amylase inactivation at pH 6. These findings point out to the important physiological role of the acidification caused by the aleurone.

• OSTI ID: 7025769
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
WHEAT
PHYSIOLOGY
ACIDIFICATION
AMYLASE
AUTORADIOGRAPHY
ENZYME ACTIVITY
GIBBERELLIC ACID
HORMONES
LABELLED COMPOUNDS
METHIONINE
SULFUR 35
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERS
EVEN-ODD NUCLEI
GLYCOSYL HYDROLASES
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY ACIDS
ISOTOPES
LACTONES
LIGHT NUCLEI
LILIOPSIDA
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
NUCLEI
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIOISOTOPES
SULFUR ISOTOPES
551001* - Physiological Systems- Tracer Techniques