Heat shock inhibits. alpha. -amylase synthesis in barley aleurone without inhibiting the activity of endoplasmic reticulum marker enzymes

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.2.506· OSTI ID:5923532

Sticher, L; Biswas, A K; Bush, D S; Jones, R L ^[1]

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1. Univ. of California, Berkeley (USA)

The effects of heat shock on the synthesis of {alpha}-amylase and on the membranes of the endoplasmic reticulum (ER) of barley (Hordeum vulgare) aleurone were studied. Heat shock, imposed by raising the temperature of incubation from 25{degree}C to 40{degree}C for 3 hours, inhibits the accumulation of {alpha}-amylase and other proteins in the incubation medium of barley aleurone layers treated with gibberellic acid and Ca{sup 2+}. When ER is isolated from heat-shocked aleurone layers, less newly synthesized {alpha}-amylase is found associated with this membrane system. ER membranes, as indicated by the activities of NADH cytochrome c reductase and ATP-dependent Ca{sup 2+} transport, are not destroyed by heat stress, however. Although heat shock did not reduce the activity of ER membrane marker enzymes, it altered the buoyant density of these membranes. Whereas ER from control tissue showed a peak of marker enzyme activity at 27% to 28% sucrose (1.113-1.120 grams per cubic centimeter), ER from heat-shocked tissue peaked at 30% to 32% sucrose (1.127-1.137 grams per cubic centimeter). The synthesis of a group of proteins designated as heat-shock proteins (HSPs) was stimulated by heat shock. These HSPs were localized to different compartments of the aleurone cell. Several proteins ranging from 15 to 30 kilodaltons were found in the ER and the mitochondrial/plasma membrane fractions of heat-shocked cells, but none of the HSPs accumulated in the incubation medium of heat-shocked aleurone layers.

• OSTI ID: 5923532
• Journal Information: Plant Physiology; (USA), Vol. 92:2; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMYLASE
INHIBITION
HYPERTHERMIA
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
BARLEY
CELL MEMBRANES
ENDOPLASMIC RETICULUM
BODY TEMPERATURE
CELL CONSTITUENTS
CEREALS
ENZYMES
GLYCOSYL HYDROLASES
GRASS
HYDROLASES
LILIOPSIDA
MAGNOLIOPHYTA
MEMBRANES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
SYNTHESIS
560200* - Thermal Effects