Identification and purification of a novel 120-kDa protein that recognizes the cAMP-responsive element
Journal Article
·
· Journal of Biological Chemistry; (USA)
OSTI ID:7025539
- Purdue Univ., West Lafayette, IN (USA)
The TGACGTCA (CRE) motif required for function by a number of cellular (somatostatin, enkephalin, alpha-human chorionic gonadotropin) and viral (Ad5 E1A-inducible, HTLV-1 TAX-inducible) genes is the site of interaction of multiple sequence-specific complexes. A protocol has been developed for the fractionation and purification of these activities. We report here the purification from HeLa nuclear extracts of a novel 120-kDa polypeptide which by Southwestern blots, gel retardation, and UV cross-linking assays displays CRE-specific binding. The CRE-affinity purified 120-kDa protein displays properties distinct from those of the 43-kDa CREB/ATF polypeptide. The 120-kDa protein is readily phosphorylated in vitro by protein kinase C but not by protein kinase A, suggesting that this molecule may mediate cellular signals distinct from the cAMP-responsive pathway. In vitro transcription-complementation assays utilizing the purified 120-kDa protein failed to transactivate the cAMP-responsive somatostatin promoter suggesting that the mode of action of this 120-kDa polypeptide may require an activation step distinct from the cAMP-signaling pathway.
- OSTI ID:
- 7025539
- Journal Information:
- Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 265:6; ISSN JBCHA; ISSN 0021-9258
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
CROSS-LINKING
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
FRACTIONATION
HELA CELLS
KINETICS
MOLECULAR WEIGHT
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
TRANSCRIPTION
TRANSFERASES
ULTRAVIOLET RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CELL NUCLEI
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
CROSS-LINKING
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
FRACTIONATION
HELA CELLS
KINETICS
MOLECULAR WEIGHT
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SEPARATION PROCESSES
TRANSCRIPTION
TRANSFERASES
ULTRAVIOLET RADIATION