Effects of a radiation-induced. cap alpha. -thalassemia on the production of multiple forms of hemoglobins in fetal mice
Embryonic hemoglobins in ..cap alpha..-thalassemic heterozygotes and normal fetuses were compared to study the effects of the deficient ..cap alpha.. chain on the synthesis of hemoglobins in the nucleated embryonic erythrocytes derived from the fetal yolk sac. Acrylamide gel electrophoresis showed that less hemoglobin Ell (..cap alpha../sub 2/y/sub 2/) was formed in ..cap alpha..-thalassemic heterozygotes between 12/sup 1///sub 2/ and 14/sup 1///sub 2/ days of gestation. Quantitation of in vitro synthesis between 11/sup 1///sub 2/ and 13/sup 1///sub 2/ days of gestation also showed that Ell was synthesized less rapidly in ..cap alpha..-thalassemic fetuses. In contrast, the synthesis of Elll (..cap alpha../sub 2/z/sub 2/) was higher in ..cap alpha..-thalassemic than in normal fetuses at 12/sup 1///sub 2/ and 13/sup 1///sub 2/ days of gestation. Measurements of the synthesis of individual chains in El (x/sub 2/y/sub 2/) and Ell showed that x chain synthesis was normal and that ..cap alpha.. chain synthesis was deficient in ..cap alpha..-thalassemic fetuses at 11/sup 1///sub 2/ and 12/sup 1///sub 2/ days of gestation. Thus, there is still no proof for close linkage of x- and ..cap alpha..-chain genes in chromosome 11. Differences in the electrophoretic patterns of embryonic hemoglobins of ..cap alpha..-thalassemic and normal fetuses can be explained by normal synthesis of x chains, deficient synthesis of ..cap alpha.. chains, and a higher affinity of z than y for the reduced amount of ..cap alpha.. chain present in the nucleated embryonic erythrocytes of ..cap alpha..-thalassemic mice.
- Research Organization:
- Oak Ridge National Lab., Tenn. (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 7025456
- Report Number(s):
- CONF-780638-1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Murine alpha thalassemia: effects on development and hemoglobin synthesis
Expression of embryonic hemoglobin genes in mice heterozygous for. cap alpha. -thalassemia or. beta. -duplication traits and in mice heterozygous for both traits
Related Subjects
550901 -- Pathology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DISEASES
ELECTROPHORESIS
ERYTHROCYTES
FETUSES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GLOBIN
HEMIC DISEASES
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SYMPTOMS
SYNTHESIS
THALASSEMIA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES