Embryonic hemoglobins in. cap alpha. -thalassemia mice
Embryonic hemoglobins in heterozygous ..cap alpha..-thalassemic and normal fetuses were compared to study the effects of the deficient ..cap alpha.. chain on the synthesis of hemoglobins in the nucleated embryonic erythrocytes derived from the fetal yolk sac. Visual inspection of embryonic hemoglobins following acrylamide gel electrophoresis suggested that less hemoglobin EII (..cap alpha../sub 2/y/sub 2/) was formed in ..cap alpha..-thalassemic heterozygotes between 12/sup 1///sub 2/ and 14/sup 1///sub 2/ days of gestation. Quantitation of in vitro synthesis between 11/sup 1///sub 2/ and 13/sup 1///sub 2/ days of gestation confirmed that EII was synthesized less rapidly in ..cap alpha..-thalassemic fetuses. In contrast, the synthesis of EIII (..cap alpha../sub 2/z/sub 2/) was higher in ..cap alpha..-thalassemic than in normal fetuses at 12/sup 1///sub 2/ and 13/sup 1///sub 2/ days of gestation. Measurements of synthesis of individual chains in EI (x/sub 2/y/sub 2/) and EII showed that x-chain synthesis was normal and that ..cap alpha..-chain synthesis was deficient in ..cap alpha..-thalassemic fetuses at 11/sup 1///sub 2/ and 12/sup 1///sub 2/ days of gestation. There is still no proof for close linkage of x- and ..cap alpha..-chain genes in chromosome 11. Differences in the electrophoretic patterns of embryonic hemoglobins of ..cap alpha..-thalassemic and normal fetuses can be explained by normal synthesis of x chains, deficient synthesis of ..cap alpha.. chains, and a higher affinity of z than y for the reduced amount of ..cap alpha.. chain present in the nucleated embryonic erythrocytes of ..cap alpha..-thalassemic mice.
- Research Organization:
- Oak Ridge National Lab., Tenn. (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6850476
- Report Number(s):
- CONF-780638-2
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CHROMOSOMAL ABERRATIONS
ERYTHROCYTES
FETUSES
GENETIC CONTROL
GLOBIN
GLOBULINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MAMMALS
MICE
MOLECULAR BIOLOGY
MUTATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEST CONTROL
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
RODENTS
SYNTHESIS
VARIATIONS
VERTEBRATES