Effect of dietary iron on the turnover rate of rat liver xanthine oxidase

Journal Article · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7024702

Cherry, D M; Amy, N K

Previous studies in this lab have shown that hepatic xanthine oxidase (XO), a molybdo-enzyme, increases in response to a low iron diet. In addition, molybdenum-deficient and low protein diets cause non-heme iron accumulation in the liver. In order to determine whether iron affects the turnover rate of hepatic XO the authors used the double isotope technique of Arias et al to measure the turnover of XO. They fed two groups of rats complete AIN semi-purified diets containing either 5 or 35 ppm iron for 14 days. The rats remained on their respective diets 10 additional days, during which time the two isotopic forms of L-leucine were administered. Although the XO activity was 25% higher in the rats fed the low iron diet, there were no differences in either the amounts of cross reacting material or the turnover rates of the hepatic XO between the two groups. Thus, the difference they see in heptatic XO activity is not due to changes in turnover rate or enzyme concentration as measured by cross reacting material.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 7024702
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
DIET
DIGESTIVE SYSTEM
DOUBLE LABELLING
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GLANDS
IRON
ISOTOPES
LABELLING
LEUCINE
LIVER
MAMMALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
RADIOISOTOPES
RATS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES