Absorption of enzymatically active sup 125 I-labeled bovine milk xanthine oxidase fed to rabbits

Journal Article · Tue May 01 04:00:00 EDT 1990 · Journal of Agricultural and Food Chemistry; (USA)

DOI:https://doi.org/10.1021/jf00095a015· OSTI ID:6172162

Rzucidlo, S J ^[1]; Zikakis, J P ^[2]

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1. ICI Americas Inc., Wilmington, DE (USA)
2. Univ. of Delaware, Newark (USA)

Rabbits fed a regular laboratory diet supplemented with a high-fat milk containing xanthine oxidase (XO) were studied to determine the presence of active XO in the blood. A pilot feeding study, where rabbits consumed a high-fat diet containing xanthine oxidase, showed a correlation between dairy food consumption and XO activity in the blood. Antibody to dietary XO was also found. In a second study, rabbits were fed ad libitum the high-fat milk and blood serum samples were tested weekly for XO activity. No elevation in serum XO activity was found. A third study showed that serum XO activity was increased when rabbits were force fed the high-fat milk. The final study consisted of force feeding {sup 125}I-labeled XO to one rabbit to ascertain whether the observed increase in serum XO was due to dietary or endogenous XO. Isoelectric focusing of sera collected from the test rabbit strongly suggested that at least a portion of the serum XO contained the radioactive label. This is the first direct evidence showing the uptake of dietary active XO from the gut.

• OSTI ID: 6172162
• Journal Information: Journal of Agricultural and Food Chemistry; (USA), Journal Name: Journal of Agricultural and Food Chemistry; (USA) Vol. 38:5; ISSN JAFCA; ISSN 0021-8561
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD SERUM
BODY FLUIDS
CATTLE
COWS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FOOD
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MILK
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PURINES
RABBITS
RADIOISOTOPES
RUMINANTS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
XANTHINES