Fatty acylation of murine Ia alpha, beta and invariant chains
Post-translational modification of MHC Class II molecules may influence their subsequent fate in further post-translational processing or in cell trafficking and expression in a recognizable form at the cell surface. Radiolabelling of lymphoid cells with (/sup 3/H)-palmitate permits the detection of covalently linked fatty acid chains on Ia alpha and beta glycopeptides and on the invariant chain (Ii). The attached fatty acid is stable to SDS-polyacrylamide gel electrophoresis, and is released in a chloroform/methanol extractable form by reagents which disrupt ester linkages. Fatty acid linkages to alpha and beta chains are sensitive to alkaline hydrolysis in KOH/methanol, and are therefore identified as hydroxyesters. The fatty acid linkage to invariant chain is susceptible to mild neutral hydrolysis in hydroxylamine or KOH/methanol, and this lability indicates that it is a thioester. Mobility of released radiolabelled material in thin layer chromatography verifies that the radiolabel remains in fatty acid. Fatty acylation has been noted in I-A and I-E molecules from all MHC haplotypes tested. Class I molecules were also found to be fatty acylated. None of the fatty acid linkages were of the amide type.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO
- OSTI ID:
- 7024701
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
ACYLATION
LYMPHOCYTES
MOLECULAR STRUCTURE
COVALENCE
ELECTROPHORESIS
HEXADECANOIC ACID
MICE
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques