Fatty acylation of murine Ia. cap alpha. ,. beta. , and invariant chains

Journal Article · Tue Apr 15 00:00:00 EST 1986 · J. Immunol.; (United States)

OSTI ID:6937186

Simonis, S; Cullen, S E

Labeling of murine spleen cells with (/sup 3/H)palmitate followed by analysis of immunoprecipitated Ia molecules indicated that Ia ..cap alpha..- and ..beta..-chains and their associated invariant chain contain covalently bound fatty acid. This modification is present in I-A and I-E molecules and has been found in all haplotypes examined. The /sup 3/H-label was not dissociated from the glycoproteins by detergents or under the denaturing conditions of SDS-polyacrylamide gel electrophoresis. The fatty acid linked to I/sub i/ is released by treatment with neutral hydroxylamine, which indicates thioester linkage. The acylation of ..cap alpha..- and ..beta..-chains appears to involve attachment of palmitoyl groups via an ester linkage sensitive to alkaline hydrolysis. The radioactive species released from the isolated chains by treating with KOH/methanol co-migrated with palmitic acid and palmitic acid methyl ester on thin-layer chromatography.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 6937186
• Journal Information: J. Immunol.; (United States), Vol. 136:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IMMUNOGLOBULINS
ACYLATION
ANTIGEN-ANTIBODY REACTIONS
DYNAMIC FUNCTION STUDIES
ELECTROPHORESIS
ESTERS
GLUCOPROTEINS
LABELLED COMPOUNDS
LIPIDS
MICE
SPLEEN CELLS
THIN-LAYER CHROMATOGRAPHY
TRITIUM
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMICAL REACTIONS
CHROMATOGRAPHY
GLOBULINS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques