Effects of three oxidizing biocides on Legionella pneumophila serogroup 1

Journal Article · Tue Mar 01 04:00:00 EST 1988 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7024695

Domingue, E L; Tyndall, R L; Mayberry, W R; Pancorbo, O C

A study was conducted to determine the bactericidal effects of ozone and hydrogen peroxide relative to that of free chlorine on Legionella pneumophila serogroup 1. In laboratory batch-type experiments, organisms seeded at various densities were exposed to different concentrations of these biocides in demand-free buffers. Bactericidal effects were measured by determining the ability of L. pneumophila to grow on buffered charcoal-yeast extract agar supplemented with ..cap alpha..-ketoglutarate. Ozone was the most potent of the three biocide, with a greater than 99% kill of L. pneumophila occurring during a 5-min exposure to 0.10 to 0.30 ..mu..g of O/sub 3/ per ml. The bactericidal action of O/sub 3/ was not markedly affected by changes in pH or temperature. Concentrations of 0.30 and 0.40 /sup +/g of free chlorine per ml killed 99% of the L. pneumophila after 30- and 5-min exposures, respectively. A 30-min exposure to 1000 ..mu..g of H/sub 2/O/sub 2/ per ml was required to effect a 99% reduction of the viable L. pneumophila population. However, no viable L. pneumophila could be detected after a 24-h exposure to 100 or 300 ..mu..g of H/sub 2/O/sub 2/ per ml. Attempts were made to correlate the biocidal effects of O/sub 3/ and H/sub 2/O/sub 2/ with the oxidation of L. pneumophila fatty acids. These tests indicated that certain biocidal concentrations of O/sub 3/ and H/sub 2/O/sub 2/ resulted in a loss or severe reduction of L. pneumophila unsaturated fatty acids.

• Research Organization: East Tennessee State Univ., Johnson City (USA)
• OSTI ID: 7024695
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 54:3; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
CHLORINE
ELEMENTS
HALOGENS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
LEGIONELLA PNEUMOPHILA
MICROORGANISMS
NONMETALS
OXYGEN COMPOUNDS
OZONE
PEROXIDES
SENSITIVITY
TOXICITY