Species differences in 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and biotransformation in fish

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Fundam. Appl. Toxicol.; (United States)

DOI:https://doi.org/10.1016/0272-0590(88)90304-1· OSTI ID:7024673

Kleeman, J M; Olson, J R; Peterson, R E

Rainbow trout, yellow perch, carp, bluegill, largemouth bass, and bullhead were treated with graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 1, 5, 25, or 125 micrograms/kg) or vehicle, ip. The lethal potency of TCDD tended to be greater in yellow perch, carp, and bullhead than in the other three species (LD50 80 days post-treatment, 3-5 versus 10-16 micrograms/kg, respectively). All species treated with the highest dose of TCDD (125 micrograms/kg) displayed a latency period of 1-4 weeks prior to death; longer latency periods were produced by lower lethal doses. Effects of TCDD treatment on body weight were both species-dependent and dose-dependent. Fin necrosis was observed in all fish species; however, cutaneous hemorrhage was observed only in TCDD-treated perch, carp, and bluegill, and cutaneous hyperpigmentation only in TCDD-treated carp and largemouth bass. Gallbladder bile was analyzed for TCDD and its metabolites 7 days after fish were injected with (/sup 14/C)TCDD (60 micrograms/kg, ip). At least three TCDD metabolites in addition to the parent compound were found in the gallbladder bile of all six species. In addition, the retention time of the major biliary TCDD metabolite (determined by HPLC) was similar in all species except yellow perch. Beta-Glucuronidase treatment of the bile from largemouth bass and bluegill suggested that at least two of the TCDD metabolites were glucuronide conjugates. Thus, species differences exist in the lethal potency, signs of overt toxicity, and biotransformation of TCDD among freshwater fish.

• Research Organization: Univ. of Wisconsin, Madison (USA)
• OSTI ID: 7024673
• Journal Information: Fundam. Appl. Toxicol.; (United States), Journal Name: Fundam. Appl. Toxicol.; (United States) Vol. 10:2; ISSN FAATD
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL VARIABILITY
CARBON 14 COMPOUNDS
DIOXIN
DOSE-RESPONSE RELATIONSHIPS
FISHES
GENETIC VARIABILITY
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLITES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
POLLUTION
TOXICITY
TRACER TECHNIQUES
VERTEBRATES
WATER POLLUTION