Measurement of glucose utilization by Pseudomonas fluorescens that are free-living and that are attached to surfaces

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:7023702

Fletcher, M

The assimilation and respiration of glucose by attached and free-living Pseudomonas fluorescens were compared. The attachment surfaces were polyvinylidene fluoride, polyethylene, and glass. Specific uptake of (/sup 1/C)glucose was determined after bacterial biomass was measured by (1) microscopic counts or (2) prelabelling of cells by providing (/sup 3/H)leucine as substrate, followed by dual-labelling scintillation counting. The glucose concentration was 1.4, 3.5, 5.5, 7.6, or 9.7 ..mu..M. Glucose assimilation by cells which became detached from the surfaces during incubation with glucose was also measured after the detached cells were collected by filtration. The composition of the substratum had no effect on the amount of glucose assimilated by attached cells. Glucose assimilation by attached cells exceeded that by free-living cells by a factor of between 2 and 5 or more, and respiration of glucose by surface-associated cells was greater than that by free-living bacteria. Glucose assimilation by detached cells was greater than that by attached bacteria. Measurements of biomass by microscopic counts gave more consistent results than those obtained with dual-labelling, but in general, results obtained by both methods were corroborative.

• Research Organization: Univ. of Warwick, Coventry, England
• OSTI ID: 7023702
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 52:4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
BACTERIA
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COUNTING TECHNIQUES
DOUBLE LABELLING
GLUCOSE
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LEUCINE
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHYSIOLOGY
PSEUDOMONAS
SACCHARIDES
SCINTILLATION COUNTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE