Ozone (O/sub 3/)-induced augmentation of eicosanoid metabolism in cultured epithelial cells derived from bovine trachea

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7022215

Leikauf, G D; Driscoll, K; Weideman, P; Lippmann, M

Epithelial damage and inflammation have been implicated in ozone-induced airway hyperresponsiveness. Because O/sub 3/ is relatively insoluble and highly reactive, initiating effects of this compound are likely limited to the epithelium. To examine eicosanoid metabolism following exposure to 0.1-10.0 ppm O/sub 3/, epithelial cell monolayers derived from bovine trachea were alternately exposed to O/sub 3/ and culture medium for 2h by rotating on inclined platforms. After control exposures, basal release rates determined by RIA were: PG(Prostaglandin) E/sub 2/ = 93.0(19.6), PGF/sub 2..cap alpha../=4.0(1.3), 6kPGF/sub 1..cap alpha../=4.9(0.9), Tx (Thromboxane) B/sub 2/=3.9(0.9), LT(Leukotriene)B/sub 4/=0.6(0.4)pg/cm/sup 2/ x h (mean (SE), n=6 to 22). After 0.1 ppm release of PGF/sub 2..cap alpha../ and 6kPGF/sub 1..cap alpha../ increased to 35.5(6.5) and 14.0(1.1)pg/cm/sup 2/ x h, while PGE/sub 2/ decreased to 76.5 (20.7). Thus, the F/sub 2..cap alpha..//E/sub 2/ ratio changed from 3.9(control) to 46.4% (0.1 ppm). Higher O/sub 3/ levels produced dose-dependent increases in cyclooxygenase product release with maximal rates occurring at 4.1 ppm (PGE/sub 2/ = 1170(314), PGF/sub 2..cap alpha../=26.9(8.2), 6kPGF/sub 1..cap alpha../=28.5(6.7)). Lipoxygenase products were much lower and unchanged by O/sub 3/ concentrations < 4.1 ppm. At 4.1 ppm, however, LTB/sub 4/ rates did increase to 4.3(0.9). After 10 ppm, all rates declined along with a decline in cell viability (98.8 to 93.0%). These findings suggest that environmentally relevant levels of O/sub 3/ augments cyclooxygenase and, at higher concentrations, lipoxygenase metabolism.

• Research Organization: New York Univ. Medical Center, NY
• OSTI ID: 7022215
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism &amp; Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CATTLE
CELL CULTURES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
EICOSANOIC ACID
ENZYMES
EPITHELIUM
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
OZONE
PROSTAGLANDINS
RESPIRATORY SYSTEM
RUMINANTS
TISSUES
TRACHEA
VERTEBRATES